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KMID : 0613820140240020167
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Journal of Life Science
2014 Volume.24 No. 2 p.167 ~ p.172
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Optimization of Extraction Conditions for Bakkenolide B from the Leaves of Petasites japonicus by Using Response Surface Methodology
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Kim Jung-Hun
Kim Yong-Min
Chung Hun-Sik
Choi Young-Whan
Lee Kyoung-Pil
Im Dong-Soon
Lee Young-Guen
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Abstract
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Optimal conditions for extraction of bakkenolide B from Petasites japonicus leaves were determined by using response surface methodology. A second-order Box-Behnken design representing three extraction temperatures (80, 100, 120℃), three extraction times (30, 45, 60 min), and three solvent pH’s (5, 7, 9) was executed. The efficiency of the extraction conditions was defined using the β-hexosamidase assay by comparing both the bakkenolide B content and its anti-allergic activity expressed as extract inhibition on degranulation. The response surface plot described for the bakkenolide B content showed that the maximum content was predicted as 121.6 μg/g with extraction conditions of 127.1℃, 46.6 min, and pH 7.7. Extraction temperature and time were important factors in determining bakkenolide B content. Using regression analysis, correlation between the inhibition effect of mast cell degranulation and bakkenolide B content was found to be low.
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KEYWORD
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Anti-allergy, bakkenolide-B, optimum extraction conditions, Petasites japonicus, response surface methodology
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